rabbit polyclonal anti mat2a antibody (Danaher Inc)
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rabbit polyclonal anti mat2a antibody
Rabbit Polyclonal Anti Mat2a Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 20242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+mat2a+antibody/pm34284075-85-94-99?v=Danaher+Inc
Average 99 stars, based on 20242 article reviews
Rabbit Polyclonal Anti Mat2a Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 20242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+mat2a+antibody/pm34284075-85-94-99?v=Danaher+Inc
Average 99 stars, based on 20242 article reviews
rabbit polyclonal anti mat2a antibody - by Bioz Stars,
2026-08
99/100 stars
Images
1) Product Images from "MiR-26b-5p-modified hUB-MSCs derived exosomes attenuate early brain injury during subarachnoid hemorrhage via MAT2A-mediated the p38 MAPK/STAT3 signaling pathway."
Article Title: MiR-26b-5p-modified hUB-MSCs derived exosomes attenuate early brain injury during subarachnoid hemorrhage via MAT2A-mediated the p38 MAPK/STAT3 signaling pathway.
Journal: Brain research bulletin
doi: 10.1016/j.brainresbull.2021.07.014
Figure Legend Snippet: Fig. 4. MAT2A was a direct target of miR- 26b-5p. A. StarBase3.0 (http://starbase.sysu.edu.cn/) was used to predicted the targeting sites of miR- 26b-5p and MAT2A. The wild-type and mutant sequences of MAT2A are shown in the figure. B. The relative luciferase activity was tested with wild-type and mutant-type of MAT2A, respec tively. C. The miR-26b-5p mimic or miR-26b-5p inhibitor was transfected into cells and the protein expression of MAT2A was analyzed by Western blotting. GAPDH was used as an in ternal reference. N = 6, *P < 0.01.
Techniques Used: Mutagenesis, Luciferase, Activity Assay, Transfection, Expressing, Western Blot
Figure Legend Snippet: Fig. 5. Effect of regulating MAT2A expression on oxyHb-induced PC12 cells. PC12 cells were stimulated with 50 ng/mL oxyHb (cell density was 1 × 106) for 6 h. The cells were then incubated with exosomes at a concentration of 100 μg/mL, and the overexpression vector of MAT2A was transfected into the cells. A. The protein expression of MAT2A was analyzed by Western blotting. B. MTT assay was used to detected cell proliferation. C. Apoptosis of PC12 cells were detected by flow cytometry. D. Western botting was used to detected the protein expression of COX-2, MCP-1 and iNOS. GAPDH was used as an invariant internal control for calculating protein-fold changes. N = 6, *P < 0.01.
Techniques Used: Expressing, Incubation, Concentration Assay, Over Expression, Plasmid Preparation, Transfection, Western Blot, MTT Assay, Flow Cytometry, Control
Figure Legend Snippet: Fig. 6. MiR-26b-5p-modified exosomes inhibited p38 MAPK/STAT3 signaling pathway to alleviate oxyHb-induced cell injury. PC12 cells were stimulated with 50 ng/mL oxyHb (cell density was 1 × 106) for 6 h and incubated with exosomes at a concentration of 100 μg/mL. Then, cells were transfected with miR-26b-5p inhibitor alone or together with si-MAT2A, respectively. A. Western botting was used to detect the protein expression of MAT2A as well as the phosphorylation levels of p38 MAPK and STAT3 proteins. B. MTT assay was used to detected cell proliferation. C. The protein expression of COX-2, MCP-1 and iNOS was analyzed by Western botting. GAPDH was used as an internal reference. N = 6, *P < 0.01.
Techniques Used: Modification, Incubation, Concentration Assay, Transfection, Western Blot, Expressing, Phospho-proteomics, MTT Assay
Figure Legend Snippet: Fig. 7. MiR-26b-5p-modified exosomes attenuate early brain injury in SAH rats. The animal model of SAH was established by the classical occipital cistern secondary injection method. Thirty SD rats were divided into five groups on average. The cerebellar medullary cistern of rats in sham operation group was injected with normal saline. A. Relative expression of miR-26b-5p was detected by RT-qPCR. B. Western botting was used to detect the protein expression of MAT2A as well as the phosphorylation levels of p38 MAPK and STAT3 proteins. C. Behavioral and neurological functions of SAH rats were evaluated 24 h after modeling. D. Brain edema content in SAH rats was detected 24 h after modeling. E. The protein expression of COX-2, MCP-1 and iNOS was detected by Western botting. GAPDH was used as an invariant internal control for calculating protein-fold changes. N = 6, *P < 0.01.
Techniques Used: Modification, Animal Model, Injection, Saline, Expressing, Quantitative RT-PCR, Western Blot, Phospho-proteomics, Control
